Next Generation Sequencing
Course description:
This course sheds light on the next generation sequencing technologies that have a wide area of
applications and analyzes several properties of biological systems. NGS is a revolutionary
technology in the biological sciences. With its ultra-high throughput, scalability, and speed, NGS
enables researchers to perform a wide variety of applications and study biological systems at a
level never before possible. Today’s complex genomic research questions demand a depth of
information beyond the capacity of traditional DNA sequencing technologies. Next-generation
sequencing has filled that gap and become an everyday research tool to address these questions.
This course will deal with diverse types of tests, analysis and visualization techniques that may be
used in the analysis of diverse types of NGS techniques.
Recommended Books:
1. Next Generation Sequencing – 2018 Methods and Protocols by Dr. Steven R. Head Dr. Phillip Or- doukhanian Dr. Daniel R. Salomon. Springer
2. Next-generation Sequencing: Current Technologies and Applications. Canister Academic Press
3. Clinical Applications for Next-Generation Sequencing. Urszula Demkow Rafal Ploski.
Elsevier
Course Learning Outcomes:
After the course, the students will apply different concepts of NGS analysis on practical problems.
They would be able to grasp the idea of high throughput sequencing and their application in various
domains.
Assessment system:
Quizzes 10-15%
Assignments 5-10%
MSE 30-40%
ESE 40-50%
Lecture Plan:
S.NO. Lecture Topic Quizzes Assignments
1. Overview of Genetic Testing and Sequencing
Technologies
2. What is Genetic Testing?
The Promise of Genetic Testing and Sequencing Technologies
3. Clinical Genetic Testing
Overview of Genetic Testing Amino Acids and Proteins
4. Traditional Cytogenetics Microarray Diagnostics
5. Biochemical Genetic Tests Diagnostic Genetic Testing
6. Reproductive Genetic Testing 2
7. Sequencing Technologies Sequencing Approaches
8. Polymerase Chain Reaction Sanger Sequencing Sequencing by Synthesis
9. Raw Data Analysis
Variant Identification
10. Clinical Linkage: Diagnostic Genetic Testing 3
11. Emerging Areas in Genetic Testing and Analysis
12. Non-coding Variants DNA Methylation Testing
13. Improving Variant Classification Complex Trait Variants
14.Determining Risk in Complex Traits
15.Long Read Sequencing
16. Single Cell Sequencing