Fundamentals of HPLC and LC-MS Techniques
Basics of Chromatography
General Principles, Retention, Column Efficiency, Selectivity, Peak Symmetry, Resolution, Chromatographic Techniques
Chromatographic Separation Principles
Normal Phase Chromatography, Reversed Phase Chromatography, Hydrophilic Interaction Chromatography, Chiral Separations, Size Exclusion Chromatography, Ion Exchange Chromatography
High Performance Liquid Chromatography
Chromatographic Separation Process, Column, Pumps, Detectors, Injectors, Mobile Phases, Solvents for Sample Preparation, Quantitative and Qualitative Analysis Strategies, HPLC troubleshooting
Theoretical and practical aspects of LC-MS
Basics of mass spectrometry; the mass spectrometer: source, analyzer and detector; electron impact (EI) ion sources, magnetic sector analyzer the mass spectrum; isotopic peaks and their use; mass spectrometry and molecular formula; high resolution mass; the nitrogen rule; the hydrogen deficiency index; fragmentation; types of fragmentation; cleavage; McLafferty rearrangement; organic molecules typical fragmentations; limits of the EI source; chemical ionization (CI), electrospray ionization (ESI), matrix assisted laser desorption (MALDI); quadrupole, ion trap, orbitrap, TOF; tandem mass spectrometry.
Quantification and Quality of Analytical Data
Peak Height and Peak Area, Calibration Methods (External Standard Method, Internal Standard Method, Standard Addition, Normalization),Validation (Analytical Procedure, Accuracy, Precision, Specificity, Limit of Detection, Limit of Quantification, Linearity and Range, Robustness, System Suitability, Adjustment of Chromatographic Conditions.
About the Course Instructor
Dr. Hammad Ullah obtained his PhD in ‘Nutraceuticals, Functional foods, and Human health” in 2023 at the Department of Pharmacy, University of Naples Federico II in liaison with the Department of Analytical and Food Chemistry, University of Vigo Spain. The research activity focuses mainly on the comprehensive investigation of natural products, with the final aim of the development of new food supplement and functional food ingredients. The scientific activity is documented by more than 50 peer-reviewed articles and 4 book chapters. He is currently an Editorial Board Member of BMC Complement. Altern. Med.,J. Med. Food, and Discover Life. His scientific contributions are recognized by several research awards including ‘MEDWELL Award for Best PhD Thesis (2023)’; ‘European Phytochemical Society (PSE)- Dra. Mariola Macías Award (2023)’; and ‘Young Researcher Award at MONASH INITIATE 2022’. In addition, he is a regular member of ‘American Chemical Society’; ‘Society for Medicinal Plant and Natural Product Research (GA)’; ‘Royal Society of Chemistry (MRSC)’; ‘International Natural Product Sciences Taskforce (INPST)’; ‘Phytochemical Society of Europe (PSE)’; ‘IUPHAR Mediterranean Group of Natural Products Pharmacology’.
Dr. Hammad Ullah, PhD, MRSC
Department of Pharmacy, School of Medicine and Surgery
University of Naples Federico II,Via D. Montesano 49, 80131 Naples, Italy