Techniques in Biotechnology
For industrial professionals
Course Description:
Techniques for DNA isolation and manipulation plays an important role in modern day biotechnology.These techniques of biotechnology with different combination can be useful in curing a particular disease, finding biomarkers of the disease and also involved in forensics. In this course all the important and modern biotechnology techniques are described with particular focus on its application and feasibility.
Key Outcomes:
• Cloning and Expression
• Blotting and Hybridization
• DNA Markers
• Specific topics for techniques discussions
• Latest developments in Biotechnology techniques
Course Objectives:
To introduce and familiarize students with different techniques used in the field of biotechnology, its practical applications with pros and cons along with the specific requirement of techniques in
different lab and industrial processes
Learning Outcomes:
After completing this course student should be able to:
Understand role of different biotechnology techniques, their practical applications, principle and core steps to perform these techniques in lab and industry.
Course Descriptions:
Week 1-2
Cloning and expression: Introduction; Types of vectors; Recent developments in vectors and
expression system; Chromosomal integration; Phage display libraries; PCR (Standard protocol,
Types and Uses); Protein fractionation: Gel Electrophoresis (Agarose, SDS-PAGE, Pulse field),
HPLC, Pulse field).
Week 3-4
Blotting, hybridization: Identification of macromolecules by Southern, Northern, Western
blotting and Colony hybridization; ELISA; Chemical synthesis of DNA; Gene probes; Sequencing
of DNA; Sequencing of proteins; Protein targeting; Transport and translocation; Protein
Purification.
DNA Markers: DNA markers such as RFLP, RAPD, AFLP and VNTR
Week 5-8
Specific topics: Transgenic mouse; Monoclonal antibodies; Glycoprotein analysis; RNA
interference; DNA Chip Technology.
Latest Developments: Recent advances and future trends in techniques related to
biotechnology along with scenario based practical applications of above-mentioned
techniques
Who Should attend?
Biology scientists
Masters and graduate students seeking knowledge about principles, applications and stpes
involved in various biotechnology techniques.
Industrial professionals
Lab managers
Lab Technicians
Lab Scientists and Researchers
PhD Scholars
Masters’ students acquiring hands on experience of Biotechnology techniques.